Phagocytosis in Plant Protoplasts
نویسندگان
چکیده
منابع مشابه
Studies on Isolated Plant Protoplasts
Isolated tomato fruit protoplasts have been observed to take up both tobacco mosaic virus and ferritin by the process of pinocytosis. These studies have involved electron microscopic observations on thin sections of suitably fixed and embedded material. These electron microscopic studies have also shown that very close association exists between the nucleus and chloroplasts in these proto plas...
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Chloroplasts can be isolated from higher plants directly following homogenization; however, the resulting yield, purity, and intactness are often low, necessitating a large amount of starting material. This protocol is optimized to produce a high yield of pure chloroplasts from isolated Arabidopsis protoplasts. The two-part method is a simple, scaled-down, and low-cost procedure that readily pr...
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The electrical fusion technique of Zimmermann and Scheurich (1981 Planta 151: 26-32) has been used to fuse mesophyll protoplasts of Avena, Zea, Vigna, Petunia, and Amaranthus. Electrical fusion proves to be a simple, effective, and general fusion technique that can be controlled to form either dikaryons or large multinucleate fusion bodies. In addition, we show that Vigna mesophyll protoplasts ...
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After 1 hour, exogenous deoxyribonucleic acid was degraded within a culture medium at 25 C (pH 6) containing protoplasts of Daucus carota L. var. sativa. Low temperature incubation (1 C) or the addition of 45 millimolar sodium citrate to the medium eliminated DNase activity for at least 4.5 hours. This DNase activity was not reduced at pH 7 or 9, nor by addition of 200 millimolar adenosine 5'-t...
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ژورنال
عنوان ژورنال: Cell Structure and Function
سال: 1978
ISSN: 0386-7196,1347-3700
DOI: 10.1247/csf.3.25